Alleles are listed in the DNA plus orientation, AKA positive strand orientation as reported by the Illumina array utilized. The positive DNA strand orientation is by convention read from 5' → 3' direction. The minus or negative orientations is 3' → 5' and represents the complementary DNA strand. Since there is no standardized orientation commonly employed in the literature, some mutations are better known as reported in the minus direction. MTHFR 677TT is actually a negative strand name, and normalized to plus is actually 677AA. See the Orientation/Ambigous Flip FAQ for more information.
Articles in this section
- Does the new StrateGene DNA kit evaluate the APOE gene status?
- Why are the reference alleles different from what I see in the literature, for example, A for T or C for G for MTHFR C677T?
- How exact is the information regarding kinetics and a variant?
- Are their other ways genes can mutate besides SNPs?
- How did you determine which SNPs to include?
- Are there other ways genes can mutate besides SNPs?
- What specific types of evidence did you look at when determining which SNPs to include?
- What is CEU?
- What if I work with patients, or I am largely of different racial background than CEU and I want to determine the frequency of an allele?